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Braz. j. microbiol ; 42(3): 825-834, July-Sept. 2011. ilus, tab
Article in English | LILACS | ID: lil-607511

ABSTRACT

A new Streptomyces sp. IF 5 was isolated from the feather dumped soil and found to have a tremendous keratinase activity. The strain enabled the degradation of the chicken feathers very effectively in 60 h. The 16S rRNA sequence of 1474 bp long was submitted to the National centre for Biotechnological information. The keratinolytic activity in the culture medium was 1181 U/ml. The release and analyses of sulphydryl groups in the culture medium evident the degradation activity by the Streptomyces sp. IF 5. The idea of the present study was to use the degraded chicken feathers as the substrate for the growth and cultivation of microorganisms. We have designed a very economical culture medium that includes the usage of some basal salts alone and degraded chicken feathers (10 g/l). The results of the specific growth rate of the tested microbes confirm the usage of the new designed medium for microbial culturing.


Subject(s)
Animals , Base Sequence , Clinical Enzyme Tests , Environmental Microbiology , Food Analysis , Food Microbiology , Genetics, Microbial , Culture Media/isolation & purification , RNA Stability , Streptomyces/isolation & purification , Chickens , Enzyme Activation , Food , Food Samples , Methods , Methods
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